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Center for Health Sciences
Biochemistry & Microbiology
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Faculty & Staff

Dr. Earl BlewettFranklin R. Champlin
Associate Professor of Microbiology
Adjunct Associate Professor of Forensic Microbiology
Professor Emeritus of Biological Sciences and Adjunct
   Professor of Veterinary Medical Research (Mississippi
   State University)

frankrc@okstate.edu
918.561.8490

Education | Professional Experience | Research Expertise | Publications

Education

Institution and Location                                 Degree                Conferred              Major

Oklahoma State University, Stillwater, OK              B.S.                       1975                Microbiology
Oklahoma State University, Stillwater, OK              Ph.D.                     1981                Microbiology

Professional Experience

1975-1981       
Graduate Teaching Assistant (1975 -77), Graduate Research Assistant (1977-80), and Faculty Lecturer (1980-81), Department of Microbiology, Oklahoma State University

1981-1982        
Postdoctoral Research Associate, Department of Medicinal Chemistry, University of Maryland at Baltimore

1982-1983
Research Associate, Inherited Disease Unit of the Center for Molecular Medicine, Oklahoma College of Osteopathic Medicine and Surgery

1983-2006
Assistant Professor (1983-89), Associate Professor (1989-97), Professor of Microbiology (1997-06), Department of Biological Sciences, Mississippi State University

1993-2006
Biological Safety Officer (Regulatory Compliance Office), Office of Vice President for Research, Mississippi State University

1994-present 
Adjunct Professor of Veterinary Medical Research, College of Veterinary Medicine, Mississippi State University

2003 
Visiting Scientist, Department of Biochemistry and Microbiology, Center for Health Sciences, Oklahoma State University

2006-present
Visiting Associate Professor (2006-07), Associate Professor of Microbiology (2007–present), Department of Biochemistry and Microbiology, Center for Health Sciences, Oklahoma State University

2007-present
Adjunct Associate Professor of Forensic Microbiology, Department of Forensic Sciences, Center for Health Sciences, Oklahoma State University

2007-present
Professor Emeritus of Microbiology, Department of Biological Sciences, Mississippi State University.

Research Expertise

Bacterial pathogenesis research in my laboratory has consistently focused on the cell envelope physiology of gram-negative bacteria representing both animal and human pathogens.  It has featured investigations of the involvement of membrane lipid and lipopolysaccharide composition in cell envelope permeability to lipophilic antibiotics and antiseptics, the process of  acquisition of adaptive resistance to antibiotics, the molecular genetic basis of capsulation and its effect on outer surface function, cell surface lipoprotein content, and the properties of cell surface hydrophobicity and charge.  Particular emphasis has been directed toward Pseudomonas aeruginosa, Burkholderia multivorans, and members of the family Pasteurellaceae pathogenic in humans and animals of agricultural importance.

Publications

BOOKS AND CHAPTERS:

Grula, E.A., R.L. Burton, R. Smith, T.L. Mapes, P.Y.K. Cheung, S. Pekrul, F.R. Champlin, M. Grula, and B. Abegaz.  1978.  Biochemical basis for entomopathogenicity of  Beauveria bassiana, p. 192 -216.  In C.M. Ignoffo (ed.), Proc. of the First Joint US/USSR Conference on the Production, Selection and Standardization of Entomopathogenic Fungi of Project V(01.0705), Microbiological Control of Insect Pests.  Amer. Soc. Microbiol. and NSF.  Washington, D.C.

Champlin, F.R. 2005.  Bacterial Cultivation Course Theory and Laboratory Handbook.  National Archive Publishing Co., Ann Arbor, MI.

RESEARCH PUBLICATIONS:

Champlin, F.R., and E.A. Grula.  1979.  Noninvolvement of beauvericin in the entomopathogenicity of Beauveria bassiana.  Appl. Environ. Microbiol.  37:1122-1126.

Champlin, F.R., P.Y.K. Cheung, S. Pekrul, R.J. Smith, R.L. Burton, and E.A. Grula.  1981.  Virulence of Beauveria bassiana mutants for the pecan weevil.  J. Econ. Entomol.  74:617-621.

Champlin, F.R., H.E. Gilleland, Jr., and R.S. Conrad.  1983.  Conversion of phospholipids to free fatty acids in response to acquisition of polymyxin resistance in  Pseudomonas aeruginosa.  Antimicrob. Agents Chemother. 
24:5-9.

Gilleland, H.E., Jr., F.R. Champlin, and R.S. Conrad.  1984.  Chemical alterations in cell envelopes of Pseudomonas aeruginosa upon exposure to polymyxin:  a possible mechanism to explain adaptive resistance to polymyxin.  Can. J. Microbiol.  30:869-873.

Darnell, K.R., M.E. Hart, and F.R. Champlin.  1987.  Variability of cell surface hydrophobicity among Pasteurella multocida somatic serotype and Actinobacillus lignieresii strains.  J. Clin. Microbiol.  25:67-71.

Champlin, F.R., M.E. Hart, and K.R. Darnell.  1988.  Low propensity for poultry isolates of Pasteurella multocida to acquire adaptive resistance to oxytetracycline.  Avian Dis.  32:478-482.

Hart, M.E., and F.R. Champlin.  1988.  Susceptibility to hydrophobic molecules and phospholipid composition in Pasteurella multocida and Actinobacillus lignieresii.  Antimicrob. Agents Chemother. 
32:1354-1359.

Gilleland, L.B., H.E. Gilleland, J.A. Gibson, and F.R. Champlin.  1989.  Adaptive resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa.  J. Med. Microbiol.  29:41-50.

Thies, K.L., and F.R. Champlin.  1989.  Compositional factors influencing cell surface hydrophobicity of Pasteurella multocida variants.  Curr. Microbiol.  18:385-390.

Champlin, F.R., and M.E. Hart.  1990.  Cell envelope impermeability to daptomycin in Pseudomonas aeruginosa and Pasteurella multocida.   Curr. Microbiol.  21:367-372.

Swords, W.E., C.-C. Wu, F.R. Champlin, and R.K. Buddington.  1993.  Postnatal changes in selected bacterial groups of the pig colonic microflora.  Biol. Neonate.  63:191-200.

Fuller, C.A., P.J. Brignac, and F.R. Champlin.  1993.  Phospholipid fatty acid ester composition of Pasteurella multocida and Actinobacillus lignieresii.  Curr. Microbiol.  27:237-240.

Morris, C.M., A. George, W.W. Wilson, and F.R. Champlin.  1995.  Effect of polymyxin B nonapeptide on daptomycin permeability and cell surface properties in Pseudomonas aeruginosa, Escherichia coli, and Pasteurella multocida.  J. Antibiotics. 48:67-72.

Conrad, R.S., C. Galanos, and F.R. Champlin.  1996.  Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of  Pasteurella multocida.  Vet. Res. Commun. 20:195-204.

Ryals, P.E.,  M.N. Nsofor, J.M. Watt, and F.R. Champlin.  1998.  Relationship between serotype A encapsulation and a 40-kDa lipoprotein in  Pasteurella multocida. Curr. Microbiol. 36:274-277.

Arif, M., and F.R. Champlin.  1998.  Adaptive acquisition of novobiocin resistance in  Pasteurella multocida strains of avian origin.  Vet. Res. Commun.  22:445-455.

Champlin, F.R., C.E. Patterson, F.W. Austin, and P.E. Ryals.  1999.  Derivation of extracellular polysaccharide-deficient variants from a serotype A strain of Pasteurella multocida.  Curr. Microbiol. 38:268-272.      

Wilson, W.W., M.M. Wade, S.C. Holman, and F.R. Champlin.  2001. Status of methods for assessing
bacterial cell surface charge properties based on zeta potential measurements.  J. Microbiol. Methods. 43:153-164.

Swiatlo, E., F.R. Champlin, S.C. Holman, W.W. Wilson, and J.M. Watt.  2002.  Contribution of choline-binding proteins to cell surface properties of Streptococcus pneumoniae.  Infect. Immun.
70
:412-415.

Labrie, J., S. Rioux, M.M. Wade, F.R. Champlin, S.C. Holman, W.W.Wilson, C. Savoye, M. Kobisch, M. Sirois, C. Galarneau, and M. Jacques.  2002.  Identification of genes involved in biosynthesis of Actinobacillus pleuropneumoniae serotype 1 0-antigen and biological properties of rough mutants.  J. Endotoxin Res.  8:27-38.
               
Champlin, F.R., T.R. Shryock, C.E. Patterson, F.W. Austin, and P.E. Ryals.  2002.  Prevalence of a novel capsule -associated lipoprotein among Pasteurellaceae pathogenic in animals.  Curr. Microbiol. 
44
:297-301.

Watt, J.M., M.M. Wade, S.C. Holman, W.W. Wilson, D.E. Keil, S.B. Pruett, M. Jacques, and F.R. Champlin.  2003. Influence of serotype A capsulation on cell surface physiologic factors in Pasteurella multocida.  Coll. Surf. B: Biointerfaces. 28: 227-238.

Watt, J.M., E. Swiatlo, M.M. Wade, and F.R. Champlin.  2003.  Regulation of capsule biosynthesis in serotype A strains of Pasteurella multocida.  FEMS Microbiol. Lett. 225:9-14.

Reeks, B.Y., F.R. Champlin, D.B. Paulsen, D.W. Scruggs, and M.L. Lawrence.  2005.  Effects of sub-MIC antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus.  Can. J. Vet. Res. 69:1-10.

Champlin, F.R., M.L. Ellison, J.W. Bullard, and R.S. Conrad.  2005.  Effect of outer membrane permeabilisation on intrinsic resistance to low triclosan levels in Pseudomonas aeruginosa.  Int. J. Antimicrob. Agents.  26:159-164.

Nsofor, M.N., P.E. Ryals, and F.R. Champlin. 2006.  Subcellular distribution of Plp-40, a lipoprotein in a serotype A strain of Pasteurella multocida.  Biochim. Biophys. Acta.  1760:1160-1166.

Ellison, M.L., A.L. Roberts, and F.R. Champlin.  2007.  Susceptibility of compound 48/80-sensitized Pseudomonas aeruginosa to the hydrophobic biocide triclosan.  FEMS Microbiol. Lett.  269:295-300.

Ellison, M.L., and F.R. Champlin.  2007.  Outer membrane permeability for nonpolar antimicrobial agents underlies extreme susceptibility of Pasteurella multocida to the hydrophobic biocide triclosan.  Vet. Microbiol. 124:310-318.

Claybom, A.B., S.N. Toofan, and F.R. Champlin. 2011. Influence of methylation on the antibacterial properties of triclosan in Pasteurella multocida and Pseudomonas aeruginosa variant strains. J. Hosp. Infect. 77:129-133.

Bullard. J.W., F.R. Champlin, J. Burkus. S.Y. Millar, and R.S. Conrad. 2011. Triclosan-induced modification of unsaturated fatty acid metabolism and growth in Pseudomonas aerufinosa PAO1. Curr. Microbiol. 62:697-702.

Ruskoski, S.A., J. W. Bullard, and F.R. Champlin. 2012. Cell envelope lipid composition of Burkholderia multivorans variant strains. Appl. Environ. Microbiol. In preparation.