Oklahoma State University Center for Health Sciences
Oklahoma State University Center for Health Sciences
Biological Research Safety - Training

Biological Safety Training (initial and as needed)

For each trainee, the Principal Investigator (PI) or other supervisor shall print the Training Documentation Form and indicate on the form which items below must be studied before the PI signifies by signature that the training is completed. The PI or other supervisor is responsible for documenting all training provided to personnel working on infectious agent and/or recombinant DNA projects. The training must be provided at least prior to the beginning of the work, when there are any changes to the work, equipment, personal protective gear, etc. and refresher training as determined by the PI or other Supervisor.

  • Biosafety in Microbiological Laboratories (BMBL) (pdf,) 5th edition, 2007, prepared by the Center for Disease Control(CDC) and National Institute of Health (NIH),. note: all should know where to find, become familiar, and follow requirements
  • Guidelines for Research Involving Recombinant DNA Molecules by NIH. note: all who are involved with r-DNA research should know where to find, become familiar and follow requirements
  • Biosafety Modules by the Center for Disease Control (pdf.) note: [sections required as determined by PI/Supervisor]:
    • Introduction and Principles, pages 1 – 12
    • BSL-1 (Biosafety Level 1,) pages 13 – 26
    • BSL-2 (Biosafety Level 2,) pages 13 – 49
    • BSL-3 (Biosafety Level 3,) pages 13 – 62
    • BSL-4 (Biosafety Level 4,) pages 13 - 78
    • BSCs (Biological Safety Cabinets,) pages 79 – 90
    • Centrifuges, pages 91 – 95
    • Shipping Biological  Specimens, pages 96 – 112
    • Biosafety Manuals, pages 113 – 114
    • Decontamination, pages 115 – 135
    • Biological Waste, pages 136 – 140
    • Medical Surveillance, pages 141 – 150
    • Emergency Response, pages 151 - 153
  • Experimental specific protocols, lab Biosafety Manual and procedures.  Note: training provided by PI/Supervisor; repeated as needed, when there are changes and as lab biosafety manual and/or funding agency requires
  • Study material safety data sheets (MSDSs) for agents being currently used in the laboratory; request from PI/supervisor (study MSDS each time a new agent is being used in the work area)
  • Laboratory Biosafety Manual (pdf,) 3rd edition, 2004, World Health Organization.  Note:  Use as a reference for hazards discussed
  • Study CHS Laboratory Emergency Response Procedures, sections 1.0, 1.3, 1.5, 1.6, 1.7 and 1.8.

Bloodborne Pathogen Training (new employee and annual refresher)

Visit Research Training Bloodborne Pathogen Training Page

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